Malaria methods and protocols [electronic resource] /
edited by Denise L. Doolan.
- Totowa, N.J. : Humana Press, c2002.
- xv, 631 p. : ill. ; 27 cm.
- Methods in molecular medicine ; 72 .
- Methods in molecular medicine ; 72. .
Includes bibliographical references and index.
Vector incrimination and entomological inoculation rates -- Epidemiological measures of risk of malaria -- Maintenance of the Plasmodium berghei life cycle -- Mouse models for pre-erythrocytic-stage malaria -- Mouse models for erythrocytic-stage malaria -- Nonhuman primate models. I. Nonhuman primate host-parasite combinations -- Nonhuman primate models. II. Infection of Saimiri and Aotus monkeys with Plasmodium vivax -- Vector analysis -- Genotyping of Plasmodium spp. Nested PCR -- Genotyping of Plasmodium falciparum. PCR-RFLP analysis -- Microsatellite analysis in Plasmodium falciparum -- Quantitation of liver-stage parasites by automated TaqMan real-time PCR -- Quantitation of liver-stage parasites by competitive RT-PCR -- Extraction and purification of Plasmodium total RNA -- Extraction and purification of Plasmodium parasite DNA -- Southern blotting of parasite DNA -- SDS-PAGE and western blotting of Plasmodium falciparum proteins -- Nested PCR analysis of Plasmodium parasites -- RFLP analysis -- Analysis of gene expression by RT-PCR -- In situ detection of RNA in blood- and mosquito-stage malaria parasites -- Purification of chromosomes from Plasmodium falciparum -- Construction of genomic libraries from the DNA of Plasmodium species -- Construction of a gene library with mung bean nuclease-treated genomic DNA -- Construction of Plasmodium falciparum lambda cDNA libraries -- Production of stage-specific Plasmodium falciparum cDNA libraries using subtractive hybridization -- Construction and screening of YAC libraries -- Episomal transformation of Plasmodium berghei -- Gene targeting in Plasmodium berghei -- Peptide vaccination -- DNA vaccination -- Assessing antigen-specific CD8+ and CD4+ T-cell responses in mice after immunization with recombinant viruses -- Assessing CD4+ helper T-lymphocyte responses by lymphoproliferation -- Limiting dilution analysis of antigen-specific CD4+ T-cell responses in mice -- Cell trafficking. Malaria blood-stage parasite-specific CD4+ T cells after adoptive transfer into mice -- Assessing antigen-specific proliferation and cytokine responses using flow cytometry -- Cytokine analysis by intracellular staining -- Assessment of antigen-specific CTL- and CD8(+)-dependent IFN-gamma responses in mice -- Assessing antigen-specific CD8+ CTL responses in humans -- Human antibody subclass ELISA -- Systemic nitric oxide production in human malaria. I. Analysis of NO metabolites in biological fluids -- Systemic nitric oxide production in human malaria. II. Analysis of mononuclear cell nitric oxide synthase type 2 antigen expression -- In vitro culture of Plasmodium parasites -- Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubator -- Hepatic portal branch inoculation -- Hepatocyte perfusion, isolation, and culture -- Inhibition of sporozoite invasion. The double-staining assay -- Inhibition of liver-stage development assay -- T-cell mediated inhibition of liver-stage development assay -- Antibody-dependent cellular inhibition assay -- Erythrocytic malaria growth or invasion inhibition assays with emphasis on suspension culture GIA -- Analysis of CSA-binding parasites and antiadhesion antibodies -- Analysis of the adhesive properties of Plasmodium falciparum-infected red blood cells under conditions of flow -- Preparation of adhesive targets for flow-based cytoadhesion assays -- Triton X-114 phase partitioning for antigen characterization -- Immunoprecipitation for antigen localization -- Field trials.
Access is restricted to subscribing institutions.
Electronic reproduction.
Mode of access: World Wide Web.
System requirements: Web browser.
Access may be restricted to users at subscribing institutions.
0896038238 (alk. paper) 9780896038233 (alk. paper) 9781592592715 (eISBN) 1592592716 (eISBN)
Malaria--Laboratory manuals.
Laboratory manuals.
Fulltext
Illustration
Index not Present
Internet Resource
Laboratory Manual
QR201.M3 / M337 2002
616.9/362/0072
W1 ME9616JM / v.72 2002 WC 25 / M237 2002
Includes bibliographical references and index.
Vector incrimination and entomological inoculation rates -- Epidemiological measures of risk of malaria -- Maintenance of the Plasmodium berghei life cycle -- Mouse models for pre-erythrocytic-stage malaria -- Mouse models for erythrocytic-stage malaria -- Nonhuman primate models. I. Nonhuman primate host-parasite combinations -- Nonhuman primate models. II. Infection of Saimiri and Aotus monkeys with Plasmodium vivax -- Vector analysis -- Genotyping of Plasmodium spp. Nested PCR -- Genotyping of Plasmodium falciparum. PCR-RFLP analysis -- Microsatellite analysis in Plasmodium falciparum -- Quantitation of liver-stage parasites by automated TaqMan real-time PCR -- Quantitation of liver-stage parasites by competitive RT-PCR -- Extraction and purification of Plasmodium total RNA -- Extraction and purification of Plasmodium parasite DNA -- Southern blotting of parasite DNA -- SDS-PAGE and western blotting of Plasmodium falciparum proteins -- Nested PCR analysis of Plasmodium parasites -- RFLP analysis -- Analysis of gene expression by RT-PCR -- In situ detection of RNA in blood- and mosquito-stage malaria parasites -- Purification of chromosomes from Plasmodium falciparum -- Construction of genomic libraries from the DNA of Plasmodium species -- Construction of a gene library with mung bean nuclease-treated genomic DNA -- Construction of Plasmodium falciparum lambda cDNA libraries -- Production of stage-specific Plasmodium falciparum cDNA libraries using subtractive hybridization -- Construction and screening of YAC libraries -- Episomal transformation of Plasmodium berghei -- Gene targeting in Plasmodium berghei -- Peptide vaccination -- DNA vaccination -- Assessing antigen-specific CD8+ and CD4+ T-cell responses in mice after immunization with recombinant viruses -- Assessing CD4+ helper T-lymphocyte responses by lymphoproliferation -- Limiting dilution analysis of antigen-specific CD4+ T-cell responses in mice -- Cell trafficking. Malaria blood-stage parasite-specific CD4+ T cells after adoptive transfer into mice -- Assessing antigen-specific proliferation and cytokine responses using flow cytometry -- Cytokine analysis by intracellular staining -- Assessment of antigen-specific CTL- and CD8(+)-dependent IFN-gamma responses in mice -- Assessing antigen-specific CD8+ CTL responses in humans -- Human antibody subclass ELISA -- Systemic nitric oxide production in human malaria. I. Analysis of NO metabolites in biological fluids -- Systemic nitric oxide production in human malaria. II. Analysis of mononuclear cell nitric oxide synthase type 2 antigen expression -- In vitro culture of Plasmodium parasites -- Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubator -- Hepatic portal branch inoculation -- Hepatocyte perfusion, isolation, and culture -- Inhibition of sporozoite invasion. The double-staining assay -- Inhibition of liver-stage development assay -- T-cell mediated inhibition of liver-stage development assay -- Antibody-dependent cellular inhibition assay -- Erythrocytic malaria growth or invasion inhibition assays with emphasis on suspension culture GIA -- Analysis of CSA-binding parasites and antiadhesion antibodies -- Analysis of the adhesive properties of Plasmodium falciparum-infected red blood cells under conditions of flow -- Preparation of adhesive targets for flow-based cytoadhesion assays -- Triton X-114 phase partitioning for antigen characterization -- Immunoprecipitation for antigen localization -- Field trials.
Access is restricted to subscribing institutions.
Electronic reproduction.
Mode of access: World Wide Web.
System requirements: Web browser.
Access may be restricted to users at subscribing institutions.
0896038238 (alk. paper) 9780896038233 (alk. paper) 9781592592715 (eISBN) 1592592716 (eISBN)
Malaria--Laboratory manuals.
Laboratory manuals.
Fulltext
Illustration
Index not Present
Internet Resource
Laboratory Manual
QR201.M3 / M337 2002
616.9/362/0072
W1 ME9616JM / v.72 2002 WC 25 / M237 2002