06108cam a22007454a 4500 1428 1428 001967120 OSt 20210628065132.0 m d cr |n| 011011s2002 njua ob 001 0 eng c 0896038238 (alk. paper) 9780896038233 (alk. paper) 9781592592715 (eISBN) 1592592716 (eISBN) (OCoLC)170845101 (OCoLC)81290779 (OCoLC)320967460 (OCoLC)658115860 COO ALUPE UNIVERSITY N$T YDXCP SCB CUV CASUM CNTRU GW5XE PUL OCLCE IDEBK pcc MYGG QR201.M3 M337 2002 W1 ME9616JM v.72 2002 WC 25 M237 2002 616.9/362/0072 21 44.44 bcl Malaria methods and protocols [electronic resource] / edited by Denise L. Doolan. Totowa, N.J. : Humana Press, c2002. xv, 631 p. : ill. ; 27 cm. text txt rdacontent computer c rdamedia online resource cr rdacarrier Methods in molecular medicine ; 72 Includes bibliographical references and index. Vector incrimination and entomological inoculation rates -- Epidemiological measures of risk of malaria -- Maintenance of the Plasmodium berghei life cycle -- Mouse models for pre-erythrocytic-stage malaria -- Mouse models for erythrocytic-stage malaria -- Nonhuman primate models. I. Nonhuman primate host-parasite combinations -- Nonhuman primate models. II. Infection of Saimiri and Aotus monkeys with Plasmodium vivax -- Vector analysis -- Genotyping of Plasmodium spp. Nested PCR -- Genotyping of Plasmodium falciparum. PCR-RFLP analysis -- Microsatellite analysis in Plasmodium falciparum -- Quantitation of liver-stage parasites by automated TaqMan real-time PCR -- Quantitation of liver-stage parasites by competitive RT-PCR -- Extraction and purification of Plasmodium total RNA -- Extraction and purification of Plasmodium parasite DNA -- Southern blotting of parasite DNA -- SDS-PAGE and western blotting of Plasmodium falciparum proteins -- Nested PCR analysis of Plasmodium parasites -- RFLP analysis -- Analysis of gene expression by RT-PCR -- In situ detection of RNA in blood- and mosquito-stage malaria parasites -- Purification of chromosomes from Plasmodium falciparum -- Construction of genomic libraries from the DNA of Plasmodium species -- Construction of a gene library with mung bean nuclease-treated genomic DNA -- Construction of Plasmodium falciparum lambda cDNA libraries -- Production of stage-specific Plasmodium falciparum cDNA libraries using subtractive hybridization -- Construction and screening of YAC libraries -- Episomal transformation of Plasmodium berghei -- Gene targeting in Plasmodium berghei -- Peptide vaccination -- DNA vaccination -- Assessing antigen-specific CD8+ and CD4+ T-cell responses in mice after immunization with recombinant viruses -- Assessing CD4+ helper T-lymphocyte responses by lymphoproliferation -- Limiting dilution analysis of antigen-specific CD4+ T-cell responses in mice -- Cell trafficking. Malaria blood-stage parasite-specific CD4+ T cells after adoptive transfer into mice -- Assessing antigen-specific proliferation and cytokine responses using flow cytometry -- Cytokine analysis by intracellular staining -- Assessment of antigen-specific CTL- and CD8(+)-dependent IFN-gamma responses in mice -- Assessing antigen-specific CD8+ CTL responses in humans -- Human antibody subclass ELISA -- Systemic nitric oxide production in human malaria. I. Analysis of NO metabolites in biological fluids -- Systemic nitric oxide production in human malaria. II. Analysis of mononuclear cell nitric oxide synthase type 2 antigen expression -- In vitro culture of Plasmodium parasites -- Automated synchronization of Plasmodium falciparum parasites by culture in a temperature-cycling incubator -- Hepatic portal branch inoculation -- Hepatocyte perfusion, isolation, and culture -- Inhibition of sporozoite invasion. The double-staining assay -- Inhibition of liver-stage development assay -- T-cell mediated inhibition of liver-stage development assay -- Antibody-dependent cellular inhibition assay -- Erythrocytic malaria growth or invasion inhibition assays with emphasis on suspension culture GIA -- Analysis of CSA-binding parasites and antiadhesion antibodies -- Analysis of the adhesive properties of Plasmodium falciparum-infected red blood cells under conditions of flow -- Preparation of adhesive targets for flow-based cytoadhesion assays -- Triton X-114 phase partitioning for antigen characterization -- Immunoprecipitation for antigen localization -- Field trials. Access is restricted to subscribing institutions. Also issued in print. Electronic reproduction. Mode of access: World Wide Web. System requirements: Web browser. Access may be restricted to users at subscribing institutions. Malaria Laboratory manuals. Laboratory manuals. lcgft Fulltext local Illustration local Index not Present local Internet Resource local Laboratory Manual local Doolan, Denise L. Print version: Malaria methods and protocols. Totowa, N.J. : Humana Press, c2002 0896038238 (DLC) 2001051656 (OCoLC)48162854 Methods in molecular medicine ; 72. http://dx.doi.org/10.1385/1592592716 MARCIVEAUT MARCIVEAUT MARCIVEAUT MARCIVEAUT Batchload MARCIVEAUT MARCIVEAUT Batchload Batchload MARCIVEAUT lcc BK PW SpringerProtocols MIT Access Only n-mit NET **See URL(s) 8 02 0 0 lcc 0 0 MLIB MLIB OS 2021-06-28 0 QR201.M3 M337 2002 28002074 2021-06-28 2021-06-28 BK PW